Do you mean VSVG pseudovirus? If so, one strategy is to optimise the transfection via variation of the DNA plasmid: transfection reagent ratio. You can also try other transfection reagents such as Lipofectamine though it is a bit expensive! Furthermore, maybe keep the cells confluent by almost 70-80% for tansfection not higher. If you use OPTIMEM media, it may also gives you a bettet titre. Hope it helps!
Dear Maryam Saleh
Pls. find the attached files, may help in your research, good luck
http://www.sciencedirect.com/science/article/pii/S0264410X15017788
http://jvi.asm.org/content/79/19/12566.full
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011265
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC304272/pdf/pnas00268-0040.pdf
http://www.bloodjournal.org/content/90/3/952.short?sso-checked=true
https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-45-64
http://www.efbs.admin.ch/fileadmin/efbs-dateien/dokumentation/Publikationen/Zimmer_B_2012_VETMIC.pdf
http://www.nature.com/articles/mto20165
Karl Maramorosch, Arthur H. McIntosh. 1994. Arthropod Cell Culture Systems CRC Press,Pp.246. ISBN0849376424, 9780849376429
Regards
Prof. Houda Kawas
What kind of cells are you using? I regularly infect BHK or Vero in around 10 15-cm dishes. After full CPE, harvest cells and virus, centrifuge to discard cell debris and concentrate virus in supernatant by ultracentrifugation (around 20,000 rpm for 1.5 hours) using 30% sucrose cushion. Resuspend the virus pellet in around 500 ul and distribute in small aliquots and store at -80. Thaw one aliquot and titrate in either of the cells mentioned before. Good luck.
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