Behnaz Heydarchi

2 Questions 12 Answers 0 Followers

Questions related from Behnaz Heydarchi

The difference of protein expression level (transient transfection) in HEK293 versus HEK293T?

I recently noticed a problem in my transient transfection. I co-transfected monoclonal antibody heavy and light chain expression vectors into 293T cells and harvested the supernatant to assess...

05 May 2017 9,984 2 View

What is the advantage of co-culturing irradiated 3T3 ms CD40L feeder cells instead of using soluble CD40L for single B cell culture?

I would like to stimulate single memory B cells in a short term culture and am wondering what is the point to co-culture 3T3 ms CD40L feeder cells to stimulate single memory B cells. Why soluble...

04 April 2014 4,396 7 View