How could I dilute 0.25% trypsin in HBSS media to get 0.05% trypsin in Hbss media and with 0.05% EDTA?

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Is there any speciation diagram Cerium nitrate in different range of pH?

I need to know the speciation of Cerium in the presence of major anions in several pH, especially Cerium Nitrate. I couldn't find any paper about it. I would appreciate it if someone could...

12 May 2024 1,971 2 View

Seeking guidance from psychologists/psychiatrists for my research on deep learning in neurodevelopmental disorders. Can anyone assist?

Dear researchers, I trust this message finds you well. I am currently engaged in research exploring the applications of deep learning algorithms in neurodevelopmental disorders. In order to enrich...

02 March 2024 8,584 1 View

What is the spectrophotometry uv-visible of cobalt(II) and cadmium(II)? Is there a special reagent for this metals?

I want to measure the concentration of cobalt(II) and cadmium(II) with UV-visible.

08 November 2023 3,911 3 View

How to find postdoc position?

post doc position in environmental engineering in AMERICA and find proffessors

07 October 2023 8,349 0 View

How can I stimulate the appetite of rats on a high-fat diet over time?

After 4 weeks, when I gave the animals a high-fat diet to induce the metabolic syndrome model, they no longer show a desire to eat, and the amount of food they consume is halved, so they do not...

02 September 2023 3,448 1 View

What is the effect of grain size on stress corrosion cracking?

In a gas turbine nozzle made of cobalt-based superalloy, the grain size is larger than normal. What are the differences between this part in terms of mechanical properties with another part that...

21 February 2022 1,986 9 View

Why can not grain size be reduced by heat treatment in cobalt base superalloys such as FSX-414?

19 February 2022 3,480 8 View

What are the appropriate methods for scenario reduction?

I am looking for a good method to reduce the number of scenarios in problems with a large number of uncertainty parameters. I implemented some of these methods in MATLAB, but unfortunately the...

23 August 2021 7,554 3 View

How to calculate the pitch factor of single layer winding?

I have a 24 slots 4 poles PM machine. There are 3 options for three-phase single-layer winding. Phase A of these 3 options are: Option #1: 1 to 6, 12 to 7, 13 to 18 and 24 to 19. (whole...

31 March 2021 2,219 0 View

Introducing methods to deal with uncertainties in power systems?

Please introduce a suitable resource for teaching methods to deal with uncertainties in power systems. (For example, Monte Carlo method, fuzzy, robust, IGDT, etc.) The suggested source should...

28 December 2020 9,908 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View