How to transfer inter-occlusal space from patients' mouth to the prosthesis laboratory?

Hi, I have a big problem with transferring the inter-occlusal space from the patients' mouth to the laboratory! Every time I receive a fabricated prosthetic dental crown or bridge from the...

31 July 2020 795 2 View

Is it possible to make a cost-effectiveness or cost-benefit analysis for the educational course of thesis preparation?

It is always come to my mind that how can I assess the pros and cons of thesis (dissertation) preparation in terms of monetary scale? Would you please let me know about related literature in this...

19 February 2020 136 6 View

How to calculate fix coefficient in non linear langmuir isotherm?

How to calculate fix coefficient in non linear langmuir isotherm?

09 February 2020 5,571 4 View

"electronic dipole and ionic dipole ?

I want to calculate dipole of one water molecule in siesta. I need to find electronic and ionic partitions.there is electric dipole (debye) in output file but electronic and ionic partitions has...

26 August 2019 1,142 2 View

Ref-prop software?

Hi dear researchers I would like to use MATLAB in order to optimization of thermodynamic cycles. I know that Refprop software can provide properties of the material. But the free version of that...

27 May 2019 1,920 1 View

Silver nano cluster synthesis problems?

Hi I tried to synthesize silver nanocluster and tested it with different concentration of DNA, AgNO3 and NaBH4, but we observe a black precipitation without any flourescent and absorbance. Is...

03 March 2018 5,549 2 View

Do you think depression affects cycle_gate?

During test of cycle_gate, we ask people to walk, then they pay attention to it and their way of walking is changed, also darkness can change it too. but that is question, whether mental illness...

29 September 2017 7,284 5 View

How to interpret very high R-square for endogenous variables in structural equation modeling?

Hi, I have a structural model including some psychological (attitudinal) constructs. The results of the SEM in AMOS show very high (up to %99) of explained variance (R-square) for my ultimate...

07 August 2017 2,575 5 View

RNA extraction from human whole blood?

02 August 2017 9,135 3 View

What bindings should be fitted in xps spectra?

07 October 2016 6,918 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View