No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How to calculate the field capacity??

28 December 2020 1,521 2 View

Which software should be used for XRD analysis in case of soils?

I am using XRD test for indentification of minerals and amount of it.

10 December 2020 8,173 4 View

How to improve total RNA concentration after transfecting the cells with the desired delivery system ?

Hello, I have been doing some transfection experiments for certain gene studies and wants to further do gene expression studies using qRT-PCR. Currently, I am using 7*10,000 cells per well for...

08 December 2020 6,981 5 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

How do I determine the units of polymerase activity for my enzyme DNA Polymerase?

One unit of DNA polymerase activity is conventionally defined as the amount of enzyme that will incorporate 10 nmol of nucleotides during a 30-min incubation. I found out about the...

04 November 2020 8,824 7 View

How to find the coordinate location of shared information in Google Drive?

Is it possible to know the coordinates of User Equipment which sent the data/information/file to another User Equipment on Google drive? Does drive has such features or option to identify the...

23 August 2020 3,315 3 View

How can I study the microstructure of carbon steel C1010 by etching process?

How can I study the microstructure of carbon steel C1010 by etching process. Which chemicals I should use for etching of carbon steel?

07 July 2020 5,106 7 View

Is there any journal who currently accepting the papers on IoT in Agriculture ?

I need information regarding publication of journal papers. The indexing of the journal should be SCI/ SCOPUS or equivalent. The topic of the journal can be IoT or automation in Agriculture or...

30 May 2020 6,794 5 View

Why is ligation reaction not giving the plasmid of interest when seen on 1.5 percent agarose gel?

I have used pet sumo vector, restriction enzymes: bsaI and BamHI, double digestion. ligation reaction mix : vector (150ng), insert (750ng) total Ligation volume 20 uL plate 1 : 20ul of ligation...

19 May 2020 8,250 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View