James Brown

5 Questions 18 Answers 0 Followers

Questions related from James Brown

What are the best cost-effective apoptosis assays?

My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...

19 September 2019 6,553 3 View

What would be the best mRNA expression marker of a senescent cell, is it p16?

We have tissue samples from old and young mice which we want to probe for correlations between markers of metabolism and senescence

01 June 2014 3,245 18 View

What is the best plasticware for keeping differentiated C2C12 cells stuck down?

Our cells differentiate really quickly in 2% horse serum + ITS and after 3 days they lift up as a plaque of cells at the edges. Will CellBind by Corning keep them stuck down?

11 April 2014 8,766 2 View

What is the most cost-effective media for culturing primary HUVECs?

I am going to perform a small study measuring responses to adipokines and have only a small funding pot allocated.

26 February 2014 4,333 6 View

When trying to induce senescence in primary fibroblasts, is there a method as quick as using H2O2 that isn't as toxic?

Our cells die even at 1uM H2O2. Any suggestions?

22 November 2013 5,972 7 View