Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Contamination when culturing primary uterine endometrial cells?

Dear everyone I'm working on the endometrium of the uterus. I need to extract endometrial cells from the endometrial tissue. Usually, I will separate the tissue into 2,3 mm pieces and incubated in...

25 November 2020 7,428 3 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

Does PROCESS v3.5 by Andrew F. Hayes with SPSS Statistics 27 on MacOS 10.15.7 work?

After installing the PROCESS v3.5 tool, I ran a mediation model 4 with three variables (N = 125). The warnings below showed up on the output window, and the status at the bottom shows, "Running...

15 November 2020 3,930 14 View

How do you deal with massless particles in SR, since they can't have four vectors?

Suppose a photon is observed to have some three velocity v in an inertial frame O, another inertial frame O' is moving away from O with another three velocity u. Is there a general way to get the...

10 October 2020 5,132 3 View

PC-3 cells: what does a neuron like/threadlike morphology indicate about the cell's condition?

I was performing wound healing assays with PC-3 cells (cultured in RPMI+10%FBS; 500,000 cells/well in 6 well plate). I seeded them for 24 hours, then applied the scratch with a 100uL pipette tip....

03 September 2020 7,664 4 View

Do you have any references on "Process to develop Child centered Social accountability"?

Dear all , I am working as the consultant for Save Children International to develop and pilot a process named " Child Centered Social accountability" in Vietnam. Does anyone know where I can get...

31 August 2020 6,297 5 View

How to build up a cell model for endometrium receptivity?

I want to conduct some research on the topic of endometrium receptivity, but I only have HEC1A cell line available. Can you please share with me the experience in using which cell line for this...

26 August 2020 2,381 1 View

What is the maximum value of PC-3 cells you can plant in a 10cm Petri Dish?

I thawed & planted the cells in a 10cm Petri dish on the morning of day one. four hours later, it looked like the first image under the microscope. on the morning of day two, it looked looked...

26 August 2020 7,175 2 View

A comprehensive guide for reproductive engineering techniques in mice?

Dear everyone, I'm doing research on infertility and need to collect sperm from male mice's caudal epididymis. Sometimes the results are good but sometimes we can not get any sperms. I have been...

20 July 2020 215 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

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28 February 2021 9,936 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View