I have treated the roots according to Phillips and Hayman (1970). How can I preserve these roots for few weeks so that I can examine it under microscope? Should I have to remove it from the trypan stain solution?
of course it is best to examine the roots sooner than later.
You might seal them on a slide with the object glass on top. seal around with some kind of wax. But you can keep them in the staining solution and in the fridge. Nevertheless the stain will loose the clearity when kept longer like this.
thank you so much prof. S.Wenkart. Highly appreciate your answer. I will try to finish my work as soon as possible.
Now I received an up-to-date answer from a postdoc from our lab as she has done it daily for months.
thank you so much for your kind help @Wenkart and S. Nemec, highly appreciated. I was really in need of some answers
Dear Husain,
I have also done similar type of work and hence I do strongly recommend the previous answers given by Madam Winkert that it would be better to do it soon rather keeping it for longer period as in spite of keeping in a freezer you might not be able to visualize the association clearly after long.
Regards and good luck.
i have done something similar, i think it is better to examine immediately and if you really must preserve, it shouldnt be more than a week, because overtime the visibility of those spores on the roots tend to reduce even when properly stained.
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