We have a protein in the buffer that contain Histidine amino acids.
by dialysis your protein against same buffer that lacks Histidine
i need to polymerize the isopropylmethacrylamide monomers to get a polymer with LCST around 37C. i read in some articles which said it depends on polymer molecular weight.
04 May 2016 3,811 4 View
i need a cancer cell line can express P-glycoproteins and also be susceptible to Doxorubicin.
03 April 2016 8,559 4 View
if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?
01 February 2016 2,639 3 View
i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...
04 May 2015 6,858 2 View
I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?
02 March 2015 903 5 View
I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.
11 December 2014 2,444 7 View
I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...
11 December 2014 1,262 9 View
There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.
03 April 2014 8,788 5 View
Biotic stress such as viral infection.
02 March 2014 1,516 7 View
Decrease of surface to volume ratio in spherical nanoparticles led to red-shift in SPR diagram. But in same size nanoparticles, shape alteration, from sphere to cube, with respect to increase of...
31 December 2013 3,318 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View