We have a protein in the buffer that contain Histidine amino acids.
by dialysis your protein against same buffer that lacks Histidine
i need to polymerize the isopropylmethacrylamide monomers to get a polymer with LCST around 37C. i read in some articles which said it depends on polymer molecular weight.
04 May 2016 3,906 4 View
i need a cancer cell line can express P-glycoproteins and also be susceptible to Doxorubicin.
03 April 2016 8,731 4 View
if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?
01 February 2016 2,767 3 View
i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...
04 May 2015 7,045 2 View
I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?
02 March 2015 1,013 5 View
I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.
11 December 2014 2,556 7 View
I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...
11 December 2014 1,394 9 View
There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.
03 April 2014 8,905 5 View
Biotic stress such as viral infection.
02 March 2014 1,625 7 View
Decrease of surface to volume ratio in spherical nanoparticles led to red-shift in SPR diagram. But in same size nanoparticles, shape alteration, from sphere to cube, with respect to increase of...
31 December 2013 3,431 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View