Is glycerol enough as a mounting medium without mixing with PBS for immunofluorescent techniques for frozen sections, and what is the difference from using glycerine in replacement?
Glycerol is the pure form and glycerin has up to 95% glycerol and thus cannot be used in place of glycerol when purity is demanded. Both names refer to the same molecule with the same formula CH2(OH)-CH(OH)-CH2(OH), propane 1,2,3-triol
Hi,
It seems that the confusion arises from the improper synonymous use of glycerol and glycerin. For more clarity, please, read here:
https://webpath.med.utah.edu/HISTHTML/MANUALS/GLYCERIN.PDF
http://applications.emro.who.int/dsaf/dsa281.pdf (p. 37)
and especially here: http://www.aciscience.org/docs/physical_properties_of_glycerine_and_its_solutions.pdf
Valeria Tananska Prof., thank you, one of these materials is very rich and offered a final resolute to the question. For the purpose of knowledge spread I want to post it here, it reads; Specimen-mounting medium ( Warren et al., 2005)
The refractive index of the cover glass–mounting medium should closely match that of the cover glass. If the refractive index of the mounting medium differs considerably from that of the cover glass, it may have an adverse affect on image quality. Mounting media should be adjusted to the pH suitable for optimal fluorescence of the fluorochrome used. For FITC it should not be lower than pH 7.2 because FITC fluorescence decreases rapidly below this pH. Oxidation and absorption of CO2 occur in stored mounting medium and thus decrease the pH of the glycerol in the mounting fluid. Therefore, pH of the mounting fluid should be at least pH 8.0, preferably between pH 8.5 and 9.0. The pH should be checked at least once a month, preferably weekly. Buffered glycerol (9 parts of glycerol to 1 part [v/v] of 0.5 M carbonate buffer [pH 9.0]) is commonly used as a cover glass mounting medium. The edges of cover glasses may be sealed with nail polish, and some preparations sealed this way may be preserved at 4 °C for weeks or months. However, this varies with the specimen and stain used. As results are not always consistent after storage, slides should be examined as soon as possible
No, they are the same. The common name of glycerol is glycerin. Like the comon name of propanone is acetone ... etc.
Ibrahim Abdel-Rahman appreciations! Your contribution is highly valued.
Dear Prof Kamoru A. Adedokun,
Many thanks. It's my pleasure.
I wish you all the best.
- Ibrahim A Abdel-Rahman
Some differences are listed.
here https://www.differencebetween.com/difference-between-glycerine-and-vs-glycerol/
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