Hello friends,
We are trying to depolarize neuronal cell by kcl, research used this way didn't elaborate much about preparing kcl. I read that we can prepare kcl medium, but I don't have a clear idea about that.
Please any one can give me a hand?
thanks in advanced
Morning,
I do not see the problem, especially if you're trained for biochemistry or pastry. Just weight the correct quantity of the salt KCl and add "distilled" water (https://www.convertunits.com/from/moles+KCl/to/grams). I do not think that 50 µM would be high enough to depolarize (rather 1mM instead), but I do not know what's your protocol.
Best
Fabien
Dear Mohamed,
If you are using a regular saline solution such as Ringer's, you can replace 50 mM NaCl by 50 mM KCl. I guess that you meant 50 mM, not 50 uM KCl. Assuming that neurons have ~ 140 mM K+ in the cytosol, then increasing extracellular K+ to 50 mM will result in a significant depolarization (~-25 mV calculated from the Nernst equation).
Hope this helps.
Fabien Tell thank you very much. Yes it is 50 mM not micro, I wrote wrong. I will edit it.
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