I am studying about Staphylococcal phage and I obtained lytic bacteriophage. Now, I want to precipitate Bacteriophage using pEG6000 with 2.5 M NaCl. How can we preparation PEG-6000 20% + NaCl 2.5 M for bacteriophage precipitation? and how can we adjust their volume (?uL of peg6000/? uL of NaCl), Could you help me, please?
Hi Candan,
You can make a single solution containing 20% PEG and 2.5M NaCl. Since NaCl is easily soluble in water, dissolve the calculated amount of NaCl in water. Weight the required amount of PEG 6000 and add small quantities of PEG in the above solution and vortex vigorously to dissolve it. If PEG is not dissolving, add few drops of conc. HCl.
how can I create drought conditions by using PEG 6000 for drought-resistant rhizobacteria in the laboratory to check their drought-resistant attributes
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