I want to make a lysis buffer for the lysis of skin samples. What should the composition of this buffer be so that my proteins will not be degraded by the proteases?
We have evaluated the wound healing potentials of different compounds at particular concentrations in laboratory animals. Now, we want to move in the direction of drug development for some topical...
31 December 2019 7,260 1 View
We want to determine the antioxidant effects of our prepared formulation of nanoparticles by DPPH method. Our formulation is having some col and different excipients/vehicles. Please also tell how...
09 October 2019 9,387 2 View
I am interested in assessing the expression of some growth factor (e.g. TGF-beta 1) or other marker (e.g. PCNA, CD31). I want to know the difference in term of significance for assessing the same...
02 March 2018 6,841 3 View
I want to measure the % wound contraction from the data of wound area by using Wilson's formula. Can anyone provide me the formula and reference for this formula?
05 June 2016 6,434 0 View
I want to mix two and more than 2 herbal drugs for the preparation of herbal formulation. Can anyone suggest or provide me the PDF file of books on herbal dose selection, their standardization in...
04 May 2016 4,409 0 View
I am interested and doing research in the field of wound healing and want to explore this area for the development of wound healing formulations faster healing. Can anyone suggest or provide PDF...
04 May 2016 6,964 1 View
I am using DMSO as a vehicle for dissolving the drug for the the topical application on wounds. Dose anyone have observed the adverse effect of DMSO on wound healing or other side effects?. What...
03 April 2016 4,246 5 View
I want to disolve drugs in DMSO and want to apply them topically on wounds, but some researchers have pointed me that it is cytotoxic even at lower concentration i.e. 10% or more. Can anyone...
03 April 2016 1,379 8 View
Citrate buffer is used for dissolving the streptozotocin for diabetes induction. Is the tri-sodium citrate used for tie preparation of citrate buffer?
03 April 2016 8,544 2 View
I have prepared some plant extracts in different solvents and among them methanolic extracts are showing best in-vitro results for antioxidant potentials. Now, I want to feed or administer these...
01 February 2016 9,935 4 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View