Can anyone provide the complete guidelines for drug development for topical formulations for wound healing?

We have evaluated the wound healing potentials of different compounds at particular concentrations in laboratory animals. Now, we want to move in the direction of drug development for some topical...

31 December 2019 7,095 1 View

How to determine the antioxidant effect of our nanoparticles by DPPH method?

We want to determine the antioxidant effects of our prepared formulation of nanoparticles by DPPH method. Our formulation is having some col and different excipients/vehicles. Please also tell how...

09 October 2019 9,228 2 View

What is the difference / significance between western blot and IHC for assessing same growth factor/other marker (e.g. TGF-beta1, alpha-SMA, VEGF)?

I am interested in assessing the expression of some growth factor (e.g. TGF-beta 1) or other marker (e.g. PCNA, CD31). I want to know the difference in term of significance for assessing the same...

02 March 2018 6,697 3 View

Can anyone suggest or provide PDF file of books on dose selection of herbal drugs and their formulation development?

I want to mix two and more than 2 herbal drugs for the preparation of herbal formulation. Can anyone suggest or provide me the PDF file of books on herbal dose selection, their standardization in...

04 May 2016 4,262 0 View

Can anyone suggest or provide PDF file of books on wound healing?

04 May 2016 6,808 1 View

Is DMSO safer for wounds?

I want to disolve drugs in DMSO and want to apply them topically on wounds, but some researchers have pointed me that it is cytotoxic even at lower concentration i.e. 10% or more. Can anyone...

03 April 2016 1,196 8 View

Does DMSO has adverse effect on topical application on wound or healing tissue?

I am using DMSO as a vehicle for dissolving the drug for the the topical application on wounds. Dose anyone have observed the adverse effect of DMSO on wound healing or other side effects?. What...

03 April 2016 4,096 5 View

Is tri-sodium citrate used for prepration of citrate buffer dissolving streptozotocin?

Citrate buffer is used for dissolving the streptozotocin for diabetes induction. Is the tri-sodium citrate used for tie preparation of citrate buffer?

03 April 2016 8,399 2 View

Can we use dried/lypholozed methanolic extracts of plants for feeding to animals?

I have prepared some plant extracts in different solvents and among them methanolic extracts are showing best in-vitro results for antioxidant potentials. Now, I want to feed or administer these...

01 February 2016 9,794 4 View

Has anyone used antibodies from Genetex company?

I want to purchase Anti CD31 antibody for rat from Genetex for IHC-P. I want to know the performance of antibodies of this company. If anyone has tested, please give the suggestions.

09 October 2015 3,713 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View