Can we find out if there is a glycoside compound in the results of column chromatography? Can glucoside compounds identified using TLC?
TLC is a simple, quick, and inexpensive procedure that gives a quick results as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound. Separate compound by using low polarity to high polarity solvents from ether to alcohols. After separation you should develop the TLC plate, and go for finding bands by comparison with the standard and on the basis of rF. Flavonoids are isolated from leaves by means of high-performance liquid chromatography ultraviolet (HPLC-UV) analysis and HPLC mass spectrometry. By using HPLC-UV, phenolics (gallic acid and quercetin) (procatechuic acid, chlorogenic acid, caffeic acid, kaempferol and ferulic acid) can be identified in extract. Structural information about the compounds can be from the retention times, the UV spectra and mass spectra without the need to isolate the individual compounds. Two flavonoids (quercetin and kaempferol) and four flavonoid glycosides (three known components, quercetin 3-O-alpha-L-arabinoside, quercetin 3-O-beta-D-glucoside and quercetin 3-O-beta-D-galactoside,and few novel compounds, kaempferol-glycoside) can be identified in the fractions.
Have you tested staining agents?
One of my favorites is Phosphomolybdic acid (PMA) which is easy to make and detects almost all functional groups. 7 g phosphomolybdic acid and dissolve it in 100 mL EtOH.
Also, Potassium Permanganate (KMnO4) is a good one for detecting stuff that acn oxidize (alcohols, alkenes). Make a mixture of 3 g KMnO4, 20 g K2CO3, 5 mL 5% aq NaOH in 300 mL H2O and enjoy life
If your glucosides have a lot of hydroxygroups, then KMnO4 would be the one to try. Hope you can spot them :)
/ Alejandro
I agree with my colleague Dr.Ravi Kant Upadhyay notations .If you know the chemical structure of your compound you can identify it by using stranded sample spotting with your mixture then from Rf you can determine your molecule.
Use TLC spraying reagents specific for Glycosides and for the column fractions also you may run it through TLC and spray with specific reagent.
Use the attached file to find the reagent.
Thank you for the answer. I will try to use the spray reagent because I do not know the type of glycoside.
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