Muthamil Iniyan

13 Questions 23 Answers 0 Followers

Questions related from Muthamil Iniyan

How to and how not to approach a supervisor for postdoctoral positions?

Experts could suggest me some tips to improve my communication to the supervisor.

15 December 2017 7,977 3 View

How do I isolate a single molecule from a mixture?

Every researchers are facing problems in isolating a single molecule (small molecules) from a mixture of crude form. The problem may be in the extraction step/ TLC / column chromatography /...

25 December 2014 4,037 4 View

Metabolome analysis for Next generation drug discovery: How is it possible?

Natural product drug discovery process depends on the purification and identification of a molecule from a mixture. The purification is being carried out based on the activity or spectral...

28 April 2014 2,224 0 View

How do you interpret LC-MS data?

How do you detect the base peak and the fragment peaks of a purified single molecule? Likewise how do you detect the base peak and the fragment peaks of a crude extract?

28 February 2014 2,951 15 View

How many numbers of secondary metabolites are produced by a bacteria and how to detect it?

Can someone share the details of number of secondary metabolite produced by a bacteria. The number may vary from species to species. Is there any references to address this question. How to detect...

03 January 2014 8,456 4 View

Can someone share the protocol for the fatty acid methyl ester analysis for the identification of Actinomycetes?

FAME profiling of bacterial cell wall hydrolysate is one of the identification tool for bacterias. Can anyone share the full protocol to extract, prepare and identify the FAME using GC-FID analysis.

26 December 2013 6,757 0 View

There are 210 antibacterials approved by FDA to date. These are under 16 classes. How do I get the list of 210 antibacterials?

I would like to know where I can find the exact list of approved antibacterial antibiotics. If you knows the the way to find this list, please share.

11 August 2013 7,809 4 View

MALDI or ESI which is suitable for small molecules?

For the mass analysis of small organic molecules, especially bioactive compounds, which ionization technique is best in Liquid Chromatography Mass Spectrometry (LC-MS)? It is evident that most of...

13 June 2013 8,023 28 View

LC-MS or GC-MS. Which would be best method for the characterization of a bioactive compound?

Please discuss the topic. Update: I have few mg of TLC purified compound from a microbial secondary metabolite. With this how can I check in both GC-MS and LC-MS? How can I identify my compound...

08 May 2013 8,911 23 View

For the purification of secondary metabolites which strategy is best to get a single molecule?

1. Crude - Column chromatography - TLC - RP.HPLC 2. Crude - TLC - Column chromatography - RP.HPLC 3. Crude - TLC - RP.HPLC 4. Crude - Column chromatography - Preparative HPLC Can anyone suggest...

11 April 2013 1,121 11 View

How to identify an actinomycete up to species level.

I did 16S rRNA sequencing and SEM analysis for the morphological characterization, but I want to know, how can I be certain that this is a new species?.

29 March 2013 7,991 3 View

How to identify whether a compound (unknown) is polar or non-polar and acidic or basic.

What is the test used for this?

29 March 2013 2,798 6 View

Can anyone suggest to me how to escape from fungal contamination in the actinomycetes laboratory?

I have problems in actinomycete culturing, maintaining and during incubation periods. I am using petri plates for the screening of actinomycetes. During the incubation periods in the incubator I...

22 March 2013 3,961 4 View