How can we avoid binding to 16S rDNA degenerate primers with chloroplast during real-time PCR?

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What ways we can develop oxidation conditions in sand columns?

I am aiming to target naphthenic acid for microbial degradation in sand column. We know that pre-oxidation via physical methds helps in degradation but I am wondering if I can achieve...

08 September 2019 2,437 3 View

Publishing series of articles?

Hi all, My question is about publishing the series articles in some kind of journals. For example, we are testing performance of constructed wetlands for different textile dyes (lets say A, B, C)...

04 May 2019 1,887 1 View

Is there any tool available that can draw dysbiosis and show change in microbial community in the form of different symbols/colors?

Hi all, I am wondering if there is any tool available to do this job? Or similar plotting. What i want to do actually is to show that the community has been changed. Before exposure and after...

07 August 2018 1,919 4 View

Organic loading rate for vertical flow constructed?

Hi all, I am trying to calculate organic loading rate for the vertical flow constructed wetland. I have COD of 493 mg/L with the flow rate 0.5 m3/d. Multiplication gives me 246.5 kg COD/m3/d. But...

05 June 2018 6,493 2 View

How to do layering of multiple images in 3d-space?

Dear all,I am writing this message to ask if you can comment on layering multiple images in 3d-space (cf attachment). I have confocal images and wants to do better presentation. Your comment would...

05 June 2017 3,707 1 View

How to find an organism’s closer relative previously annotated?

Hi all, The question is pretty straightforward, I want to find out closer relative of Juncus effusus closer relative whose genome sequence is fully annotated (Protein evidence in FASTA...

05 June 2017 8,513 6 View

How can I perform rarefaction analysis from fasta file transcriptome assembly?

Hi all, I want to do rarefaction analysis for the plant "Juncus effusus" transcriptome assembly. At the moment I have Fasta file with final assembly. I wonder how can I use this dataset? Is there...

04 May 2017 3,184 2 View

How to make tree file for phyloseq microbiome data analysis in phyloseq?

Dear All, The queation is quite straightforward. I dont have .biom file from Qiime. But I only have excel file with the sequences and OTU info. I want to know that how can i make a tree file...

02 March 2017 4,958 0 View

Selection of embedding media for plant samples to avoid detachment on hybridization slide during washing in fluorescent in situ hybridization (FISH)?

Hi all, I need expert opinions on this matter as my plant samples are detached during washing step of FISH. I used agarose as well as transparent nail polish for this purpose and to me agarose...

07 August 2016 5,594 20 View

How to check the primer binding potential with chloroplast DNA while amplifying plant endophytes?

Hi All, I want to ask if we are trying to amplify plant endophytes (specifically firmicutes) from the bulk DNA (extracted by MPbio kit), how can we check the binding of primers with chloroplast...

06 July 2016 2,884 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View