What ways we can develop oxidation conditions in sand columns?

I am aiming to target naphthenic acid for microbial degradation in sand column. We know that pre-oxidation via physical methds helps in degradation but I am wondering if I can achieve...

08 September 2019 2,309 3 View

Publishing series of articles?

Hi all, My question is about publishing the series articles in some kind of journals. For example, we are testing performance of constructed wetlands for different textile dyes (lets say A, B, C)...

04 May 2019 1,754 1 View

Is there any tool available that can draw dysbiosis and show change in microbial community in the form of different symbols/colors?

Hi all, I am wondering if there is any tool available to do this job? Or similar plotting. What i want to do actually is to show that the community has been changed. Before exposure and after...

07 August 2018 1,811 4 View

Organic loading rate for vertical flow constructed?

Hi all, I am trying to calculate organic loading rate for the vertical flow constructed wetland. I have COD of 493 mg/L with the flow rate 0.5 m3/d. Multiplication gives me 246.5 kg COD/m3/d. But...

05 June 2018 6,393 2 View

How to do layering of multiple images in 3d-space?

Dear all,I am writing this message to ask if you can comment on layering multiple images in 3d-space (cf attachment). I have confocal images and wants to do better presentation. Your comment would...

05 June 2017 3,587 1 View

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05 June 2017 8,412 6 View

How can I perform rarefaction analysis from fasta file transcriptome assembly?

Hi all, I want to do rarefaction analysis for the plant "Juncus effusus" transcriptome assembly. At the moment I have Fasta file with final assembly. I wonder how can I use this dataset? Is there...

04 May 2017 3,080 2 View

How to make tree file for phyloseq microbiome data analysis in phyloseq?

02 March 2017 4,847 0 View

Selection of embedding media for plant samples to avoid detachment on hybridization slide during washing in fluorescent in situ hybridization (FISH)?

07 August 2016 5,465 20 View

How to check the primer binding potential with chloroplast DNA while amplifying plant endophytes?

06 July 2016 2,790 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View