Ingredient in medium may be as in case of normal fungi and along with some amount of soil extract might help in growing of VAM fungi.
Ingredient in medium may be as in case of normal fungi and alongwith some amount of soil extract might help in growing of VAM fungi.
Excellent recommendation from Hani Antoun! Since centuries the direct growth of AM fungi has been tried, but no success could be achieved due to the obligate biotrophic development of these symbionts. But the germination process can be studied on suitable media. Also the infection process can be followed on these media with transformed root cultures - but the techniques is rather laborious. I also recommend the literature (published by Springer) from Declerck S., Strullu D.-G., Fortin J.A. 2005: In vitro culture of mycorrhizas. Good luck!
I agree with above recomendations but there is some AM fungi which growth without plant and transformed root in media.
Please tell us which ones, Mohsen Barin, and what growth ecxactly means in the cases you are referring to.
This might be fungi like Piriformospora indica - but these do not belong to the Glomales. These are root inhabiting endophytes - not "true" AM fungi. - But I like to learn more about anything new - so agree with Johannes Wöstemeyer, please let us know, which Genera and species you mean - thanks in advance! HWD
Hi!
I would recommend this book, it worked for me:
http://www.springer.com/life+sciences/plant+sciences/book/978-3-540-24027-3
There is also a course given yearly in Belgium that is helpful for your purposes
http://www.mycorrhiza.be/
Best
is there any evidences for synthetic media development for the growth of Mycorrhizae with out plant roots.
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