I want to search the promoter sequence of a gene or total genome.
I have a clone of 9kDa protein in pET28a, at EcoRI and HindIII. Can anyone suggest me, how can I remove the His tag?
05 June 2018 3,749 8 View
Please check the attached gel pic. Protein 1 and DNA has normal interaction. When I incubated protein 1(300nM in all lanes), protein 2(with increasing concentration 500nm to 8uM) and DNA(same...
01 February 2016 9,508 8 View
I'm using pET28a+BL21(DE3)...and my protein size is around 32kD with H-tag. I have C_His tag and N_His tag(Different clone set), but when I am going to check these proteins expression level after...
11 December 2014 6,813 12 View
Already I install this program... but when I give it for run that time I face some problem. when I write "./naccess" it show usage naccess pdb_file [-p probe_size] [-r vdw_file] [-s stdfile] [-z...
08 September 2014 3,068 5 View
I already did PCR and purified my PCR products from gel. Now I am trying to do a clone in a vector. The problem I'm facing though is wondering how long a polyA tail remains in a PCR product.
02 March 2014 4,602 4 View
Why PEG-8000? Why NaCl?
31 December 2013 5,143 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
