Avijit Das

7 Questions 13 Answers 0 Followers

Questions related from Avijit Das

How can I remove N-terminal His tag from pET28a express protein ?

I have a clone of 9kDa protein in pET28a, at EcoRI and HindIII. Can anyone suggest me, how can I remove the His tag?

06 June 2018 3,726 8 View

I need help to interpret this EMSA/GSA data..?

Please check the attached gel pic. Protein 1 and DNA has normal interaction. When I incubated protein 1(300nM in all lanes), protein 2(with increasing concentration 500nm to 8uM) and DNA(same...

02 February 2016 9,487 8 View

How can I purify my protein as soluble form?

I'm using pET28a+BL21(DE3)...and my protein size is around 32kD with H-tag. I have C_His tag and N_His tag(Different clone set), but when I am going to check these proteins expression level after...

12 December 2014 6,778 12 View

Anyone can help me about naccess...?

Already I install this program... but when I give it for run that time I face some problem. when I write "./naccess" it show usage naccess pdb_file [-p probe_size] [-r vdw_file] [-s stdfile] [-z...

09 September 2014 3,043 5 View

How long does a polyA tail remain after PCR?

I already did PCR and purified my PCR products from gel. Now I am trying to do a clone in a vector. The problem I'm facing though is wondering how long a polyA tail remains in a PCR product.

03 March 2014 4,582 4 View

Can anyone advise why do we use PEG-8000 for phage precipitation along with NaCl?

Why PEG-8000? Why NaCl?

01 January 2014 5,128 2 View

How can one get the promoter sequence of bacteriophage genome?

I want to search the promoter sequence of a gene or total genome.

10 October 2013 2,130 0 View