Can anyone advise why do we use PEG-8000 for phage precipitation along with NaCl?

More Avijit Das's questions See All

How can I remove N-terminal His tag from pET28a express protein ?

I have a clone of 9kDa protein in pET28a, at EcoRI and HindIII. Can anyone suggest me, how can I remove the His tag?

05 June 2018 3,749 8 View

I need help to interpret this EMSA/GSA data..?

Please check the attached gel pic. Protein 1 and DNA has normal interaction. When I incubated protein 1(300nM in all lanes), protein 2(with increasing concentration 500nm to 8uM) and DNA(same...

01 February 2016 9,508 8 View

How can I purify my protein as soluble form?

I'm using pET28a+BL21(DE3)...and my protein size is around 32kD with H-tag. I have C_His tag and N_His tag(Different clone set), but when I am going to check these proteins expression level after...

11 December 2014 6,813 12 View

Anyone can help me about naccess...?

Already I install this program... but when I give it for run that time I face some problem. when I write "./naccess" it show usage naccess pdb_file [-p probe_size] [-r vdw_file] [-s stdfile] [-z...

08 September 2014 3,068 5 View

How long does a polyA tail remain after PCR?

I already did PCR and purified my PCR products from gel. Now I am trying to do a clone in a vector. The problem I'm facing though is wondering how long a polyA tail remains in a PCR product.

02 March 2014 4,602 4 View

How can one get the promoter sequence of bacteriophage genome?

I want to search the promoter sequence of a gene or total genome.

09 October 2013 2,149 0 View

65% saturation of ammonium sulfate in 100 ml? (for protein precipitation)?

Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...

09 July 2024 5,508 0 View

How to disperse silverparticles in water?

I synthesize silver nanoparticles ，and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...

09 July 2024 7,335 0 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

19 June 2024 2,590 0 View

How to Precipitate of Glucose?

Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution

13 June 2024 438 0 View

How to precipitate dipeptide?

Hello I have synthesized a dipeptide in an aqueous media. Now I want to precipitate and filter it. How to precipitate it. Thank you in advance. Velmurugan S

11 June 2024 525 0 View

Download of Satellite Rainfall Estimates?

What is the easiest way to download daily precipitation data for CMORPH, PERSIAN, TRMM and CHIRPS?

02 June 2024 4,038 2 View

How to make a good recrystallization for a milky precipitates?

I have made some compounds using DMSO , the precipitates I have got had milky skeleton. I want to know the best way to get crystallin precipitate.

01 June 2024 6,879 0 View

What are these substances forming in my cell culture?

I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...

30 May 2024 5,169 2 View

How can I get an Annual Precipitation Data of a Province?

Hello forum members, I'm conducting research on vegetable oil fingerprinting. I need annual precipitation (rainfall) data for provinces in Thailand, Malaysia, and Indonesia. Specifically, I don't...

30 May 2024 6,209 2 View

Will Pluset (FSH and LH) aliquots kept in -20°C work after thawing?

Hi. I would like to know your oppinions on freezing superstimulatory hormones (namely Pluset, Calier) and keeping them in -20°C. In was using Stimufol in our lab and it was working fine when...

30 May 2024 9,497 0 View

Philippe Le Mercier

PEG and NaCl are contributing to precipitate the virions, usually achieved by incubation at low temperature. If we were not precipitating virions, they would not pellet even few hours at 10k rpm.

http://c.ask.nate.com/imgs/knsi.php/381588/11/11.gif

Avijit Das

thank u .... Philippe