What is the best place to get a custom antibody made?

I need to produce a custom antibody and I need the company to also synthesize the peptide. Does anybody have experience with a company that they liked?

01 February 2016 9,401 1 View

How do you soak your nitrocellulose membrane before transferring during a western blot?

31 December 2014 7,758 17 View

Following a bradford assay, how do you know how much protein to load onto your gel in order to get visible bands?

I am attempting to do a western blot to look for differential MMP7 expression in skin biopsies. I know the expression is differentially regulated based on immunohistochemical evidence and want to...

07 August 2014 4,528 3 View

Does anyone know about how to preserve the skin samples for use in HPLC when isolating the antimicrobial linezolid from rat?

I am interested in running human skin samples through HPLC to determine linezolid concentrations. Linezolid is an antimicrobial agent of the oxazolidinone group. I am wondering if anyone has...

06 July 2014 621 0 View

Using PCR to obtain 16s rRNA amplicons from thermally injured skin?

I am attempting to use 16s rRNA primers to amplify bacterial DNA for sequencing. Does anyone have experience using pcr to obtain amplicons? I am worried my thermocycler settings are not optimized...

02 March 2014 2,811 2 View

Can anyone help with a human PDGFR-B primer for qPCR?

I am having trouble finding a suitable primer for human PDGFR-B. Has anyone successfully used a primer and if so what was the annealing temp?

02 March 2014 9,017 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View