Bonnie C Carney

7 Questions 3 Answers 0 Followers

Questions related from Bonnie C Carney

What is the best place to get a custom antibody made?

I need to produce a custom antibody and I need the company to also synthesize the peptide. Does anybody have experience with a company that they liked?

02 February 2016 9,444 1 View

How do you soak your nitrocellulose membrane before transferring during a western blot?

Before proceeding with the transfer step, I have been soaking the nitrocellulose membrane for 10 minutes in TBST. Is this sufficient, or should it be for longer? Thanks, Bonnie

01 January 2015 7,901 17 View

How can one calculating the total body surface area in sprague dawley rats?

I am attempting to calculate TBSA in Sprague-Dawley rats based on the paper attached. I want to use Formula 3 (Vallois' formula), but they don't describe well the measurements that need to be...

10 October 2014 4,301 2 View

Following a bradford assay, how do you know how much protein to load onto your gel in order to get visible bands?

I am attempting to do a western blot to look for differential MMP7 expression in skin biopsies. I know the expression is differentially regulated based on immunohistochemical evidence and want to...

08 August 2014 4,604 3 View

Does anyone know about how to preserve the skin samples for use in HPLC when isolating the antimicrobial linezolid from rat?

I am interested in running human skin samples through HPLC to determine linezolid concentrations. Linezolid is an antimicrobial agent of the oxazolidinone group. I am wondering if anyone has...

07 July 2014 662 0 View

Using PCR to obtain 16s rRNA amplicons from thermally injured skin?

I am attempting to use 16s rRNA primers to amplify bacterial DNA for sequencing. Does anyone have experience using pcr to obtain amplicons? I am worried my thermocycler settings are not optimized...

03 March 2014 2,852 2 View

Can anyone help with a human PDGFR-B primer for qPCR?

I am having trouble finding a suitable primer for human PDGFR-B. Has anyone successfully used a primer and if so what was the annealing temp?

03 March 2014 9,058 2 View