you can deplete monocytes/macrophages using beads specific for them which are commercially available and check for contamination by Flow cytometry.

I hope this will help.

Said.

Hi

Macrophages and monocytes will be there when you isolate MSCs from cord blood, but as you continue your culturing only MSCs will proliferate and expand, whereas macrophages/monocytes are differentiated cells and they no longer proliferate or divide, and as u passage your cells only MSCs will be detached fast whereas most of the macrphages/monocytes will be retianed in the plate. Finally you can check the MSC lineage of your expanded cells by FACS or immunostaining with MSC surface markers.

I agree with Ms. Sivaraman's suggestion. Moreover, macrophages and monocytes firmly attached to the bottom of the plastic plates within 2-4 hours, and you can triturate the plate with culture medium to wash off MSC from the plate. Otherwise, continuous culturing and wait for macrophages and monocytes to die out within 2 weeks.

Simply by adding 10-7 M dexamethasone...it is very simple! Pretested FCS!