Please could someone explain to me what is happening to my sample? On the left of the plate l have a suspected pathogen (which l inoculated) and on the right l have bell pepper stigmas. I surface sterlised the stigmas with 1% sodium hypochlorite before l inoculate them. However, its appears that l have some sort of a filamentous fungi growth germinating from the right side(where the stigmas are). This is not suppose to be the case, because the idea behind the inoculation was to see if the bell pepper flowers exudates some bioactive compounds which the fungi (from the left) would be attracted to.
How can l avoid this if l were to repeat the experinment? If some fungus or other microorganisms were still on stigmas after sterilisation does thid means my solutuion of 1% of Sodium hypochlorite in 200ml was not surficient enough to sterlise the stigmas in the first place?
Thank you all
Fatna
Hi,
pleas upload pictures. This might help us all to answer your question.
Best regards
Perhaps if you show some photo of the plate, but from what you explained it means that the pepper stigmas still carries some other specific pathogen inside (like A. niger) because according to you, you 'surface sterlised' so next time use an healthy pepper stigmas
Hi guys,
Sorry l forgot to upload the photo. Here's the phot.
Thank you all for your time.@ Francesco Desiderio @Asemoloye Michael Dare
Also use 70% ethanol to ensure complete sterilization, because the chlorine evaporates in water with the passage of time. if fungus still grows this indicates intercellular infection.
with greeting.
Thank you Huda Waleed Hadi James Blodgett for your time. In future l will 70% ethanol.
- Badges
- Science method