Actually i work on visceral and subcutaniuse fat from mice and culture them with both methodes of 2d and 3d. My cells are not differentiated well. I need the exact protocol to compair with mine.
What is the most suitable NMR solvent for carbon-based materials?
25 December 2020 5,020 4 View
We have a one meter long copper pipe through which water passes at a certain speed. The outer wall of the tube is covered by the heater where all the energy of the heater is transferred to the...
21 December 2020 8,954 8 View
Hi guys. Can anyone answer the following with citing the references? 1) Relationship between adsorbent porosity and adsorption/desorption rate? 2)Relationship between porosity and adsorbent...
31 October 2020 6,323 5 View
Hi dear community, I am currently working on a project where I want to generate a 3D reconstructed model consisting of rubble piles (in consequence of a building collapse) via remote sensing. In...
10 September 2020 4,685 4 View
I already did it before but this time as the model is complicated I have faced with tis error: The surface mesh is intersecting or close to intersecting,making it difficult to create a volume...
05 September 2020 9,558 1 View
I am trying to model a fluid-structure interaction (FSI) Hyperelastic beam in COMSOL Multiphysics. The model works fine for low inlet flow velocity like 0.016 [m/s], but it gives the error below...
02 February 2020 3,394 3 View
how ferroelectricity can improve the efficiency of solar cell and what parameters are important in this relation?
01 February 2020 8,331 3 View
Hello I want to use DTI data from this site : http://insight-journal.org/midas/community/view/21. unfortunately, I do not now how extract bval and bvec file, I know that I can use MIPAV...
26 December 2019 4,281 2 View
Hello, There is a strange discrepancy in the DTI data. The b-vectors and b-values that are provided have 16 entries each. However, there are 17 volumes of diffusion data provided (They are...
26 December 2019 2,674 2 View
hello which carrier is the best of conjugation for peptide or hapten? KLH or BSA? why? Thank you for your answers
30 October 2019 4,794 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View