How can I use PCR without genomic extraction on N2a cells?

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How can I prepare Lysozyme digestion buffer for Invitrogen DNA extraction kit (Gram positive bacteria)?

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What kind of drug molecules could be fused to produce fused prodrugs?

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How bin size affects Particle Size Distribution?

Hi, I have question which I could not find an appropriate answer. By default, each laser diffraction instrument plots PSD using different bin sizes on the x-axis. This means, using default...

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Any guidelines for number of cores/problem size in parallel CFD simulations?

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Simulation and measurement results are different. What can be the reason?

I simulated a patch antenna. I saw that the best S11 value is at 5.9GHz but when I measured I saw that it works at 5.93 GHz. What can be the reason?

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How can we draw or get the centerline of a surface using Solidworks?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

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How do I increase the yield of my PCR product?

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Research on Supply Chain Management in Indian context?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to calculate the annealing temperature of a primer pair?

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01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

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What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Katie A Burnette

Sounds like you are very new to molecular biology. Talk with other folks in your lab to get your basic questions answered.

Cristina Piñeiro

Dear Hüseyin Evci,

I agree with Katie A Clark, you need some help to learn the basics of molecular biology, but regarding to your answer, typically 1uL of your samples of lysed cells should be enough.

Good luck!

Cris

Noor Idan Jarad

i agree with Katie A Clark