How can I use PCR without genomic extraction on N2a cells?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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What is the ideal efficiency for a qPCR?

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Katie A Burnette

Sounds like you are very new to molecular biology. Talk with other folks in your lab to get your basic questions answered.

Cristina Piñeiro

Dear Hüseyin Evci,

I agree with Katie A Clark, you need some help to learn the basics of molecular biology, but regarding to your answer, typically 1uL of your samples of lysed cells should be enough.

Good luck!

Cris