What is the best ITS primers for discrimination of fungi species?

Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by a few limitations, the ITS will remain as the key choice for fungal identification. I usually see the ITS 1F...

11 August 2020 3,597 10 View

How can I prepare Lysozyme digestion buffer for Invitrogen DNA extraction kit (Gram positive bacteria)?

I'd like to prepare a Lysozyme and Lysozyme Digestion Buffer (25mM Tris-HCL, pH 8.0, 2.5mM EDTA, 1% Triton X-100) for bacterial cell lysate with a final Lysozyme concentration of 20mg/mL. I need...

29 July 2020 2,818 2 View

How bin size affects Particle Size Distribution?

Hi, I have question which I could not find an appropriate answer. By default, each laser diffraction instrument plots PSD using different bin sizes on the x-axis. This means, using default...

21 October 2019 6,899 3 View

Any guidelines for number of cores/problem size in parallel CFD simulations?

Hello, I constantly submit parallel jobs in a shared HPC cluster for my OpenFOAM flow simulations and like everyone else, I have to decide how many cores I need. Our cluster runs constantly over...

11 May 2019 8,329 6 View

Simulation and measurement results are different. What can be the reason?

I simulated a patch antenna. I saw that the best S11 value is at 5.9GHz but when I measured I saw that it works at 5.93 GHz. What can be the reason?

23 September 2017 9,104 2 View

How can I use PCR without genomic extraction on N2a cells?

I have primers and i think i have to lysis the N2a cells but after that i couldn't find any protocol like how much i must add for template or other things.

06 June 2017 8,442 3 View

How can we draw or get the centerline of a surface using Solidworks?

I would like to learn drawing or getting centerline of a surface in Solidworks. I search on internet but I found limited information. I found a programs like VMTKLab.There is a section for...

12 May 2015 2,748 7 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Can anyone explain how to calculate encapsulation efficiency(Plant extract encapsulated alginate nano particles) using total polyphenol content?

Please suggest me if there are any reference materials for this topic. Thank You

30 December 2020 3,799 2 View

Does anyone have access to this Artifical urine paper by Finlayson?

Hi, I am writing a manuscript and I am looking to get a copy of the following paper below. Although there are references to the proposed urine composition in the literature I have been unable to...

29 December 2020 8,068 1 View