Greetings.
I have a question about the process of calculating the amount of solvent (in my case saline and HCl) which I should use to reach the concentration for Rat injection in my thesis research.
the drugs involved in my research are Scopolamine (for Cognitive disorder modeling) and Donepezil and Memantine (for treatment section).
I have problem about how should I calculate the amount of saline 7.2% and HCl I should use to dissolve each Drug.
For example, preferred Memantine concentration for i.p injection in our research is 20 mg/Kg, so for Rats with weighing 200 to 260 g (230 in average), the dose of 4.6 mg is suitable. we have 1 gram of Memantine. so how should I calculate so that in each 0.2 cc of the final stock of (drug+solvant), there would be the desired amount of drug (4.6 mg)?
any idea or reference for guidance would be much appreciated.
many thanks in advance.
You are far better off adjusting the injection volume based on the body weights of the individual rats so that they all receive exactly 20 mg/kg.
The common injection volume for rats is 1 mL/kg. For a 200 g rat, that is 0.2 mL. For a 260 g rat, that is 0.26 mL.
So, if you prepare your memantine solution at 20 mg/mL, you can administer an injection volume of 1 mL/kg and this will come out to be exactly 20 mg/mL.
This is far better than administering the same volume to each rat - the lighter rats would be receiving too high of a dose and the heavier rats too low of a dose.
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