Hi,
I have a problem with a hight contet of mucopolysaccharides in some molluscan samples. I tried different DNA extration procolols but I can't already remove all the molecules that inhibit PCR reactions and I'm studying other solution. I see a treatment of rice seed samples with alpha amylase to remove these type of molecules. I'd like to know if someone uses this enzyme in animal samples and what quantitive I have to use (mg/ml).
I know nothing about molluscum polysaccarides, but unless they consist mainly of starch, I dôubt that amylase will be of any help. Since PCR amplifies minute amounts of DNA, I would try reducing the amount of template in order to decrease the concentration of whatever inhibitors may be present in the DNA preparatio.
@Pierre Béguin thank you for your suggestion. I will try this way.
Best regards.
Kindly check https://www.researchgate.net/publication/13935522_Amylase_in_Pecten_maximus_mollusca_bivalves_Protein_and_cDNA_characterization_quantification_of_the_expression_in_the_digestive_gland
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