How do I select monoclones from cells grown in suspension?

08 September 2015 4,045 4 View

How can I revive DF-1 cells?

I am having difficulties in reviving the chicken embryonic fibroblast cell line DF-1. I froze down the cells in complete DMEM medium plus 5% DMSO (half of a entire T75 flask) and thawed following...

05 June 2015 4,845 2 View

Does boiling cell pellet in PAGE SDS-loading buffer efficiently release membrane-bound proteins?

03 April 2015 8,835 5 View

Does biotin labeling on RNA affect annealing?

I'm planning to synthesize biotin-labelled hairpin RNA by in vitro transcription (use biotin-UTP) and wondering if the biotin modification affects hairpin annealing (i.e. base-pairing). Thank you...

31 December 2014 1,670 4 View

How do you triphosphorylate RNA?

03 April 2014 9,772 1 View

What is the minimal length for a gene-specific RT primer?

(see attached diagram) I am doing a two-step qPCR. I incorporated different point mutations at the 3' end of my target RNA and am planning to measure the difference in RNA expression level using...

02 March 2014 9,119 6 View

Does purified RNA contain secondary structures in water?

I synthesized single-stranded RNA (~50 nt) containing complementary terminal sequence using T7 in vitro transcription and am wondering whether it folds correctly after purification (in water). Thanks!

02 March 2014 8,985 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View