How do you triphosphorylate RNA?

04 April 2014 1 10K Report

As I know, T4 polynucleotide kinase can only add one phosphate group to the 5´ -hydroxyl terminus, and chemical synthesis of 5'ppp-RNA is very expensive. IVT can achieve this but the exact sequence (especially the terminal seq) is hard to control. So I am wondering if anyone knows an alternative that can be done in the lab.

Leszek Blaszczyk

Maybe there are some nucleotide analogs bearing 5' triphosphate that you can introduce into RNA like cap analogs (i.e. ARCA) during in vitro transcription?

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