8 Questions 5 Answers 0 Followers
Questions related from Guanqun L. Liu
Hi all, Does anyone have done selection of monoclones from cells that are grown in suspension? In our case, we need to create stable THP-1 cell lines using puromycin. One specific question is that...
09 September 2015 4,087 4 View
I am having difficulties in reviving the chicken embryonic fibroblast cell line DF-1. I froze down the cells in complete DMEM medium plus 5% DMSO (half of a entire T75 flask) and thawed following...
06 June 2015 4,887 2 View
My immunoblotting failed to detect a protein of interest and I'm using the M-PER™ Mammalian Protein Extraction Reagent, which could efficiently extract both cytoplasmic and nuclear proteins...
04 April 2015 8,943 5 View
I'm planning to synthesize biotin-labelled hairpin RNA by in vitro transcription (use biotin-UTP) and wondering if the biotin modification affects hairpin annealing (i.e. base-pairing). Thank you...
01 January 2015 1,704 4 View
Except for electroporation, is there any reagent supporting protein transfection? And, which electroporation device you normally use gives good transfection efficiency (such as to HEK293T...
01 January 2015 4,722 3 View
As I know, T4 polynucleotide kinase can only add one phosphate group to the 5´ -hydroxyl terminus, and chemical synthesis of 5'ppp-RNA is very expensive. IVT can achieve this but the exact...
04 April 2014 9,806 1 View
I synthesized single-stranded RNA (~50 nt) containing complementary terminal sequence using T7 in vitro transcription and am wondering whether it folds correctly after purification (in water). Thanks!
03 March 2014 9,034 8 View
(see attached diagram) I am doing a two-step qPCR. I incorporated different point mutations at the 3' end of my target RNA and am planning to measure the difference in RNA expression level using...
03 March 2014 9,188 6 View
