How can I test for mutations in the same protein from two different cell lines?

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Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

27 November 2020 7,540 2 View

What are the alternative way to identify the stem cells present in cord blood??

commonly used practices for the identification and characterization of the kind of cell in cord blood other than Flowcytometry and reverse-transcription polymerase chain reaction (RT-PCR).

08 August 2020 6,881 3 View

What is the difference between Hex Dominant and Cut Cell Meshing methods in CFD?

Though both the methods look similar I believe there may be differences in them. What are the disadvantages of cut-cell meshing method and why is not widely used in research in comparison with...

12 July 2020 5,636 3 View

Does anyone have GS-990 DIW EIW water filtration equipment from Advantec Japan?

I tried to contact [email protected] but no response so kindly help me in this regard. We have an Advantec GS-990, it was purchased a long time ago from a Korean Collaboration. Currently,...

16 June 2020 9,890 1 View

What is the difference between anionic and cationic redox probes?

I have done Kinetics study of two different probes namely Fe(CN)63-/4- and Ru(boy)2+ on glassy carbon electrode. I kept the potential between -0.2 to 0.6 and 0.8 to 1.2 respectively. In FeCN when...

25 April 2020 3,364 10 View

Is SIMSCALE software a good to go option for visualizing and understanding academic concepts?

On what scale can SIMSCALE simulation software be helpful for students in their engineering course work comprising CFD ( Computational fluid dynamics ), thermal and structural concepts? Can...

29 March 2020 2,952 3 View

Is the Reference Intensity Ratio of Martensite(7.98) and that of Austenite (10.81), standardized against corundum, constant?

I came across the RIR value of Martensite in a paper, which is 7.98. Similarly for Austenite, which is 10.81. I want to quantify Retained Austenite in my heat treated samples. I am using Reference...

26 February 2020 9,401 2 View

Can a thermoelectric material may have negative lattice thermal conductivity?

I have calculated electronic components of thermal conductivity by wiedmanfranz law. Then the lattice thermal conductivity is derived from it. But some of those values are negative. at the end of...

26 January 2020 1,507 4 View

Anyone familiar with protein family with highly conserved C-terminal BUT very DIVERSE N- terminal, within same protein family?

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08 January 2020 1,320 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

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03 March 2021 8,920 3 View

How to increase a model accuracy ?

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03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Divaker Choubey

If the mutation in question is not known to stabilize protein such as p53 tumor suppressor (which can be tested by using the membrane in immunoblotting) or impair binding to a protein (which can be tested by far-western assay using the membrane), the approach you indicated is the simplest one.

Michael B LoMonaco

An alternative approach is to do 2D western to identify a change in charge or MW. Not trivial unless your lab is setup for it. Good luck!

Ganesh Satyanarayana

thank you. i'll go ahead with the RT-PCR method