How to quantify the fluorescence intensity of an image using ImageJ software?

Dear All: I wanted to quantify the fluorescence intensity of the images taken by fluorescence microscopy. This can be performed by using ImageJ software. I have attached an image (TIF file) here...

04 October 2020 2,883 4 View

Hi, i have a question about atomic absorption spectrometer Analytik jena Novaa 400p. I have an error message which appears : Energy in autozero run?

I have an error message which appears in the screen of atomic absorption spectrometer Analytic jena novAA 400p.

20 April 2019 9,841 1 View

I want analyze sample of used engine oil by GC-MS, I must just dilute it in hexane or there is another treatment before analysis?

I'm working on the biodegradation of used engine oil by bacteria, I must extract it from culture and analyze it to determine the bacterial activity againt the used engine oil, then I will analyze...

09 December 2018 7,315 6 View

How can i get a curvature sensogram for single cycle kinetics in SPR?

Hi, I have been tryig to get curvature sensogram using a proteinA chip for single cycle kinetic but instead of it the sensogram raising linearly and reaching quickly to steady state just after...

11 July 2018 4,062 2 View

Is it possible to use DSMC method for Refrigerant flow in microchannel for analysis?

As by experimentally we know about the flow of Refrigerant inside the micro channel but for analysis it is possible by CFD or DSMC simulation or not.

31 May 2018 968 3 View

What is a suitable temporary adhesive which can withstand high temperature VSM (>=350C) without affecting magnetic properties being measured?

03 February 2017 3,697 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View