I want to analyse cell cycle with flow cytometry "partec " applying PI.
i used abcam protocol for staining with PI and fixation with 66% ethanol.
SSC vs FSC plot is scattered very broad . how can i overcome to this obstacle .
on the other hand how can i change software flomax for best operation and best results.
Thank you
I have problem with fsc vs ssc which doesn't dependent to pi.
Hello
In my experience, the unhealthy cells or death cells may result in broad- scattered SSC vs FSC plot. The ice ethanol was used to fixation (along with slightly shaking), and discarded before PI staining. If you do so, the plot is still scattered very broad, maybe you should use the new cell lines. I hope this suggestion would help you. Good Luck
Thank you
But i think that changing gain and amplification may be helpful. Can you help me about theas seting.
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