How can i separate Quantum dots by size instead of Chromatography and Dialysis Bag Method ?

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What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

Can we fit a gaussian Fit on a LIBS Peak ?

Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...

23 July 2024 6,127 1 View

Can the MDFT mental health care model help reduce drug addiction?

This question might come up in my research on "Frustration, Fantasy, and Drug Addiction," where I'm also trying to figure out how to overcome mental health issues without turning to drugs.

30 June 2024 5,936 0 View

What is the effect of redshift on the evolution of an AGN?

AGN (Active Galactic Nuclei) are high energy galaxies powered by supermassive blackholes. The evolution of a galaxy is characterized by parameters such as luminosity and redshift. The redshift is...

25 June 2024 3,782 2 View

Which smoothening model is acceptable for processing of circular dichroism spectra?

For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks...

16 June 2024 3,997 1 View

I am carrying out MTT Assay using HeLa cells but the absorbance values are really less, can anyone please help?

I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...

12 June 2024 1,460 3 View

Can we use LIBS Peak - Ti 441.492 for the Univarate Analysis ?

I have 17 different steel samples, in which i identified a peak of Ti 441.492. But , if you look closely at photo, while some some peak are at 441.492 , some are at the slight offset. eg 441.490...

10 June 2024 8,934 2 View

Challenges in Univariate Analysis for Stainless Steel Samples using Laser Induced Breakdown Spectroscopy?

Respected Members , I have a 17 different steel sample references , with varying concentration for Minor Elements being Mo , Ni , Mn ,Si , Cr etc , i am trying to build a calibration curve with...

10 June 2024 993 0 View

Is radio emission thermally or non thermally dominated?

Thermal emission is temperature dependent. Non-thermal emission is not temperature dependent such as synchrotron emission.

29 May 2024 5,155 2 View

Normalization in LIBS Spectra ?

Hello Everyone ! I have a samples a satinless steel , I performed the LIBS Measurement with hLIBS (SciAps Z-300). Total of 12 measurements on different locations were done on the same sample...

09 May 2024 7,959 6 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

I want to remove Urea from my protein sample?

I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...

06 August 2024 1,350 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View