For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks for convolution width as an input ranging from 5 to 25. Based on the model that we choose and the convolution width, the output smoothed spectra changes and accordingly the secondary structure content varies. Thus, my question is which model is ideal and widely used in industry and why?

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