I want see exons and introns sequences in my genes but these gene are for Capra hircus and too many databases have not this organism's genome, for example Ensembl or USSC database. How can I see exons and introns sequence in vector NTI or another software or database website? What is its instruction?
Thank you
Hi, do you have your gene (DNA) and cDNA sequences of your gene? If yes, then you can compare these two sequences with each other. Anywhere that DNA does not match to cDNA is intron and other sections are exons.
Dear Ali
The Ensembl (http://ensembl.org/) gives you such possibility.
When you go into page of your interest gene you can adjust
"Sequence" according your aims.
Kind regards
If the sequence is annotated, you can go to the Text pane (top left pane in standard configuration) and look under Feature Map. There should be an Exon node there, which you can expand. You can also 'link' the graphics pane to the text pane to make it show only the features you are interested in, etc.
If exon/intron boundaries are not known a priori, then you have to compare the genomic DNA sequence to the cDNA sequence and annotate the sequence yourself. Another alternative would be to use intron prediction software.
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