How can I remove SUMO of protein?

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Ziguo Zhang

Hi Jun, You need use senp1 protein to release the sumo tag from your protein. Here is the reference about the senp1 protease.

Xu Z, Au SW: Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1. Biochem J 2005, 386:325-330.

Jun Han

Thank you very much