Ziguo Zhang

14 Questions 137 Answers 0 Followers

Questions related from Ziguo Zhang

Can VSV proteins be shared among viruses?

Dear biologists, I am wondering when we co-infect insect cells with two viruses (BacMAM viruses), only one of them containing VSV gene. Would VSV-G protein integrate onto both viruses produced...

11 November 2017 1,975 0 View

How to improve PCR fidelity while using DMSO to overcome GC rich template PCR?

Hi friends, I am trying to clone some GC rich fragments. The PCRs work well while I use 5% DMSO in the reactions, hardly any band without DMSO. The problem is that the DMSO inclusion largely...

10 October 2017 6,489 3 View

How can I find out the transcription start site (TSS) of gene CDKN1A?

Dear friends, May you let me know how can I find out the transcription start site of gene CDKN1A from any database or any public resource? Thank you. ziguo

07 July 2017 2,315 2 View

Chromosome associated RNA (caRNA), where I can find database for none coding RNA including caRNA?

Dear all, I am trying to find some sequence information about chromosome associated RNA. Please may you let me know where should head? Thank you.

07 July 2017 7,258 1 View

Coli strain for plasmids with R6Kγ origin and Reduces plasmid recombination/increases plasmid stability?

Dear friends, Does anyone knows any Coli Strain available for plasmid with R6Kγ origin (pir gene) and with reduced recombination to increase plasmid stability? I am currently experiencing a very...

12 December 2016 6,366 1 View

Any anti monomeric EGFP antibody available?

Dear All, I am aftering anti-monomeric EGFP antibody for western blotting analysis. Any friend knows where I may buy such antibody, please? Thank you.

07 July 2016 3,973 5 View

What special attention should I pay for purification of recombinant type II restriction EcoRV?

Hi experts, I need a lot EcoRV restrict enzyme for routine usage. I prepare to make by my own. As restricts are not normal protein, do I need special tricks to deal with the over-expressed enzyme...

06 June 2015 1,627 4 View

Can ProA-immunoglobulins affinity be directly used for western?

I have a ProA tagged protein in yeast. I wonder if I can use yeast crude lysate in SDS PAGE, then visualize ProA by anti-mouse IgG-peroxidase conjugate as western analysis? Or I have to do it in a...

04 April 2015 5,266 1 View

How can we increase the solubility of E. coli overexpressed protein?

We are trying to purify an artificial protein. Very good expressed, but mostly in the spun down pellet. If anyone has experience to make it a bit more soluble, with a kind of mild buffer,...

01 January 2015 5,907 8 View

How can we perform migration of peptide correctly in SDS-PAGE?

Dear All, I am trying to detect synthesised peptides with SDS-PAGE. The peptides migrate with a big difference (larger) in SDS-PAGE gel in comparison with their real molecular weight. And they...

12 December 2014 6,118 8 View

How can I decrease lysate viscosity for isolation of DNA/protein complex from cell disruption?

Dear all, I am going to isolate a DNA/Protein complex from insect cells. In protein purification practice, generally, we use nuclease during the cell disruption to destroy the released nucleic...

08 August 2014 3,473 6 View

Can anyone suggest a protein binding partner with pm affinity?

Hi, I am after protein (peptide or tag) binding partners with binding affinity in pm range. Could you suggest which protein partner I could use, please? Thank you.

07 July 2014 4,075 5 View

Which form of BirA is more active?

I am purifying BirA from coli expression, trying to do protein in vitro biotinylation. From the GF column, the protein is separated with two peaks, one is its dimerization form. My question is...

03 March 2014 5,761 1 View

How to arrest insect cell at G2/M phase?

In one of my experiments, I want to synchronize Sf9 (or any insect cell line) at G2/M phase. There is a report about insect cell arrest by yeastolate limitation. Does anyone know if...

08 August 2013 4,997 1 View