Hello!
I am doing an assay with colorectal cancer cell lines using a 96-well flat-bottom plates with 1,5% agarose and it is forming what looks like spheroids. However the problem is how to remove it from the plate to an eppendorf? We tried different options like a little spoon to try to catch the agarose and hopefully the spheroid too, or to remove it with a pipette but with the pipette it was impossible because it was "stuck" in there simply.
Can anyone help me please?
Thanks in advance!
I don't know about spheroids but I would like to tell about pipette method (may be i was used by you) that if the spheroids are large you could cut the tip(as this increase the pore size of tip) of pipette and use this this tip to remove spheroids. hopefully this will work
All the best
Like Prakash said, cut the top off a P1000 and use it to move the spheroids.
Hi Ana,
You can try plating the cells in a bigger plates and then try to cut the spheroids out under a dissection microscope.
Or you can add some more medium to the wells containing spheroids and use fine pipette tip to mix the content of the well with medium to make it less viscose so you can use pipette to remove the content of the well to a tube.
Or you should be able to add PBS to the well and mix it with content of the well and then remove all of it to a tube.
Hope it is of any help to you,
Best,
Hassan
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