Ana Luísa Ramos

3 Questions 2 Answers 0 Followers

Questions related from Ana Luísa Ramos

Can anyone tell me which can be the best concentration of DCFH-DA for ROS detection by fluorescence microscopy?

Hello fellows!I'm using HCT116 colorectal cancer cell lines, and I would like to know which could be the best concentration to detect (general) ROS with DCFH-DA. Something between 2-5µM?Thanks in...

03 March 2016 8,871 5 View

How can I remove spheroids from 96-well flat-bottom plate with agarose?

Hello! I am doing an assay with colorectal cancer cell lines using a 96-well flat-bottom plates with 1,5% agarose and it is forming what looks like spheroids. However the problem is how to remove...

10 October 2015 9,852 7 View

How can I remove spheroids from 96-well flat-bottom plate with agarose?

Hello!I am doing an assay with colorectal cancer cell lines using a 96-well flat-bottom plates with 1,5% agarose and it is forming what looks like spheroids. However the problem is how to remove...

10 October 2015 8,471 3 View