Veronica Hughes

5 Questions 4 Answers 0 Followers

Questions related from Veronica Hughes

Help with high background staining with immunofluorescence of tumors?

I am relatively new to immunofluorescence, and I am having trouble getting my assay working. I have a murine tumor that was formalin-fixed overnight and paraffin embedded. The tumor was sectioned...

10 October 2016 6,063 6 View

What is the best way to construct a microdialysis calibration curve?

Do you dilute your protein of interest in the perfusion buffer/serum/etc? Has anyone tried spiking the mouse? Thanks in advance :)

02 February 2016 4,182 1 View

Does anyone have experience transfecting either the KHT or the RIF fiborsarcoma cell lines with lipofectamine?

Any experience with plasmid concentration, efficacy, etc?

09 September 2015 3,882 0 View

Does anyone have any experience with becoming an illustrator for publications?

If anyone has, how did you learn? What software do you use? Do you do illustrations for only your own publications or do you provide your service to others?

07 July 2015 3,965 3 View

Career in science illustration?

Does anyone here work as a scientific illustrator? I'd like to ask how you got started, what software you use, and what is the best way to get your foot in the door? How many hours a week do you...

06 June 2015 4,881 2 View