Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View

How can you derive a and n parameters of the Van Genuchten equation for horticultural mediums?

Dear all, I am trying to figure out how to derive and n parameters of the Van Genuchten equation for compost samples taken from an urban farm. RETC has predefined values for these parameters for...

26 January 2020 7,843 3 View

I am trying to form microspheres with PCL and PVA using a mechanical stirrer but the polymers keeps forming clumps. What do i do to disperse them?

I prepared a 1% PVA, and dissolved 500 mg of PCL in 5 ml of DCM. Then I introduced the PCL solution into PVA while stirring at 2000 rpm for 2 hours, after which i place it on magnetic stirrer to...

15 April 2019 7,177 4 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Please, can I get a research paper or an article on lipid extraction from ogbono and egusi using chloroform-methanol method ?

I am working on a project that involves lipid extraction using chloroform-methanol as solvents. I washed the product with water-methanol and about to use a rotary evaporator for evaporation. But...

10 May 2018 4,089 3 View

What is the effect of differentating 3T3-L1 preadipocytes in differentiation cocktail for 3 versus 7 days?

I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that? Is an adipocyte grown in DM 3 days + MM 4 days...

08 December 2017 1,792 1 View

How to calculate sample size for a moderation study?

Hi, I am doing a study design and planning to do a moderation study. This is the simplest model. However, I am struggling with the calculation of sample size. How do I calculate the sample size...

23 April 2017 5,449 8 View

I want to take for the first time CHO cells in culture. How can I get 100% sterile glass Erlenmeyer flasks to use them again in the cell culture?

29 November 2015 9,661 3 View

How do I design primers that will distinguish between highly homologous transcripts?

10 August 2014 4,948 4 View

How can I get my ligation to work?

I've been attempting to subclone an insert from one vector to another by PCR amplification with primers that incorporate XhoI/EcoRI sites at the ends of the insert and then digesting the insert...

30 July 2014 4,230 6 View

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03 March 2021 5,360 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

Suggestions for using antisense oligonucleotide for brain delivery?

hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...

29 January 2021 4,455 5 View

How to find degree of deacetylation of chitosan colorimetrically?

I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...

27 January 2021 8,532 2 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

How to calculate theoretical drug loading for polymer nanoparticle and calculated nanoparticle ?

nanoparticles coated with chitosan as well uncoated nanoparticle

26 January 2021 9,053 6 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

Do polymers with different chain lengths have UV-Vis absorption peaks at different wavelengths?

I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...

06 January 2021 2,218 5 View