Stella Dozie

5 Questions 9 Answers 0 Followers

Questions related from Stella Dozie

If i culture adherent cells and after about 2 days they have not reached 70% confluence, do i just change the media or go ahead and trypsinize them?

I cultured MDA-MB-231cells in L15+ media and after 2 days the growth is still scanty. Do I just change the media or tripsinize the cells and put them in a fresh culture vessel and fresh media?

12 July 2022 5,717 1 View

I am trying to form microspheres with PCL and PVA using a mechanical stirrer but the polymers keeps forming clumps. What do i do to disperse them?

I prepared a 1% PVA, and dissolved 500 mg of PCL in 5 ml of DCM. Then I introduced the PCL solution into PVA while stirring at 2000 rpm for 2 hours, after which i place it on magnetic stirrer to...

16 April 2019 7,421 4 View

How can I redisperse gold nanoparticles after centrifuging to concentrate them?

I have tried using votex but they still remained clustered together.

11 November 2015 4,241 3 View

How can I remove paraffin from chitosan microspheres?

I prepared chitosan microspheres using liquid paraffin (W/O emulsion method). I have tried washing with n-hexane but I still can not get rid of the oil. Please what else can I do?

19 June 2014 9,307 4 View

How can I attach a thiol group to gold nanoparticles?

I need to conjugate gold nanoparticles with either a peptide or folate but need a linker between these and the gold. What ratio should I use to make the thiol react with the gold? What...

10 April 2014 7,881 6 View