Is there a technique to remove free tachyzoite antigens from a suspension of Toxoplasma tachyzoites? I've tried washing in PBS, but the free antigen appears to be entering the pellet along with the tachyzoites. What is a better way to clean whole tachyzoites?
if you are working with mice ascites fluid, as the ag are proteins, use centrifugation at low rpm, i mean 1000 or 1500 rpm, the parasite go to the bottom and upper liquid phase will have all proteins including ags...
Thank you for this suggestion. We are culturing organisms in vero cells.
If you are talking about soluble antigens from Toxoplasma gondii, I suggest freeze/thaw procedure followed by sonication. Please, see the papers from our group describing step-by-step of these protocols.
Dear Milton,
Could you inform to us what kind antigen you are expected ?
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