Can I view the image of liposomes under TEM without negative staining?

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Denis Korneev

In general, cryo-EM is better for such objects. Drying artifacts make negative staining almost useless for such "soft" structures. Dry samples without staining? No way. I'd recommend to look for a cryo-EM facility around.

Good luck!

Roger Davis

Hi Milton,

I agree with Denis. The size and shape of liposomes can be characterized by cryo-EM as it can directly visualize individual particles including their inner architecture.

https://www.creative-biostructure.com/liposome.htm