The pH of the medium affects the size and distribution of the nanoparticles. At low pH, coalescing occurs forming larger nanoparticles while at high pH value small nanoparticles are formed.
By using standard buffer solutions such as acetic or phosphate buffer system. First the pH and chemical composition of plant material /dispersion needs to be determined in which synthesis is carried out .
Biological methods in general give a greater size dispersion.
With Biological methods of nanoparticle synthesis, the pH of the solution can only be varied in a limited range. Beyond this range, stability of materials change, charge on molecules change, electrochemical potentials change etc and you could have complications.
Is the limited range of pH variation useful?
You could regulate the pH of the plant extract solution by adding external buffer of desired pH and appropriate concentration. pH variation can only be in a limited range. This has to be done with care since it can have consequences.
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