Hello Khaled Salah
I would not be knowing whether you are pertaining to plant or animal tissue culture. But if you are interested in animal tissue culture, below are a few points that I have mentioned.
When you purchase a mammalian cell line from the vendor, the vendor provides a product sheet which contains description of the cell line, storage condition, growth condition as well as the handling procedure which will include the details of the culture media to be used, subculturing procedures, media renewal as well as the cryopreservation media to be used to freeze cells for later use.
The culture media for the cell line which you are interested to culture in animal tissue culture is available commercially either in powder or liquid form. The powder may be dissolved in the required volume of sterile distilled water and filter sterilized using 0.2µm filter. The pH of the media has to be adjusted in the range of 7.2-7.4. This media may be termed as “incomplete media”. The culture media to be used for the growth and maintenance of cells in animal tissue culture is termed as “complete media” which is incomplete media supplemented with extra nutrients. The main supplement is fetal bovine serum (FBS) which is added to most of the culture media at a concentration of 10%. The FBS concentration may vary depending on the cell line. So, if you are preparing 100ml complete media, you will add 10ml FBS to 90ml incomplete media.
Some culture media in addition to FBS, also require extra nutirients to meet the growth requirements of the cells in invitro conditions. These include L-glutamine or sodium pyruvate or nonessential amino acids(NEAA) which are commercially available and may be added accordingly as mentioned in the product sheet. Some specialized reagents are also required for certain cell lines such as growth factors, cytokines, hormones, etc.
To mention a few of them, the mammalian cell lines could be purchased from the following:
1. The European Collection of Authenticated Cell Cultures (ECACC)
2. American Type Culture Collection (ATCC)
3. German Collection of Microorganisms and Cell Cultures GmbH (DSMZ)
Best.
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