How can I prepare a stock solution of papain enzyme ?

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Badreddine Mechri

Best Luck.

http://www.worthington-biochem.com/pap/PAP.pdf

Seied Mehdi Miri

Dear Shraddha

Papain is soluble in water at 10 mg/ml. Immediately prior to use, the enzyme is typically diluted in buffer containing ~5 mM L-cysteine. Activation/stabilizing agents include EDTA, cysteine, and dimercaptopropanol.

https://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/analytical-enzymes/papain.html